5I49

RNA Editing TUTase 1 from Trypanosoma brucei in complex with UTP analog UMPNPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-26
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000000
Spacegroup name	P 21 21 2
Unit cell lengths	129.380, 58.430, 66.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.403 - 1.800
R-factor	0.1747
Rwork	0.173
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hzd
RMSD bond length	0.007
RMSD bond angle	1.081
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.840
High resolution limit [Å]	1.800	1.800
Rmeas	0.056	0.427
Number of reflections	47571
<I/σ(I)>	31.75	86.4
Completeness [%]	100.0	99.4
Redundancy	13.1
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.1M Tris pH8.5, 19% PEG 3350,0.2M Lithium chloride, 1mM MgCl2. Crystals were soaked with 0.5mM UMPNPP.