5I45

1.35 Angstrom Crystal Structure of C-terminal Domain of Glycosyl Transferase Group 1 Family Protein (LpcC) from Francisella tularensis.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	38.484, 58.962, 41.646
Unit cell angles	90.00, 117.65, 90.00

Refinement procedure

Resolution	29.550 - 1.350
R-factor	0.16355
Rwork	0.162
R-free	0.20303
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB-3C4Q
RMSD bond length	0.011
RMSD bond angle	1.560
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MoRDa
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.370
High resolution limit [Å]	1.350	1.350
Rmerge	0.073	0.660
Number of reflections	36249
<I/σ(I)>	35.5	2.6
Completeness [%]	99.7	98.9
Redundancy	4.9	4.9
CC(1/2)	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	292	Protein: 10.4 mg/ml, 0.01M Tris-HCL (pH 8.3); Screen: PACT (D6), 0.1M MMT buffer (pH 9.0), 25% (w/v) PEG 1500.