5I44

Structure of RacA-DNA complex; P21 form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-03
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.989
Spacegroup name	P 1 21 1
Unit cell lengths	56.600, 68.500, 117.400
Unit cell angles	90.00, 97.50, 90.00

Refinement procedure

Resolution	500.000 - 2.621
Rwork	0.239
R-free	0.26880
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.234
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	500.000
High resolution limit [Å]	2.620
Number of reflections	49675
<I/σ(I)>	15.5
Completeness [%]	98.6
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG 3000, 0.1 M Tris 8.0, lithium sulphate