5I3D
Sulfolobus solfataricus beta-glycosidase - E387Y mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-19 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.680, 129.060, 191.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.528 - 2.160 |
| R-factor | 0.1719 |
| Rwork | 0.170 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gow |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.633 |
| Data reduction software | xia2 (0.4) |
| Data scaling software | Aimless |
| Phasing software | PHENIX (1.10.1_2155) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.528 | 2.220 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.190 | 0.400 |
| Number of reflections | 119882 | |
| <I/σ(I)> | 11.2 | 2.3 |
| Completeness [%] | 97.0 | 61 |
| Redundancy | 10.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.25 | 291 | PEG 4000, sodium acetate, ammonium acetate |
