5I36
Crystal structure of color device state A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 77.15 |
| Detector technology | CCD |
| Collection date | 2013-08-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.530, 69.131, 70.106 |
| Unit cell angles | 98.19, 97.34, 99.74 |
Refinement procedure
| Resolution | 33.583 - 5.123 |
| R-factor | 0.1371 |
| Rwork | 0.134 |
| R-free | 0.16500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gbi |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.278 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX ((1.10_2152)) |
| Refinement software | PHENIX ((1.10_2152)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.600 | 5.330 |
| High resolution limit [Å] | 5.100 | 5.240 |
| Rmerge | 0.902 | |
| Number of reflections | 4725 | |
| <I/σ(I)> | 9.77 | |
| Completeness [%] | 96.4 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 30 mM sodium cacodylate, 50 mM magnesium acetate, 50 mM ammonium sulfate, 5 mM magnesium chloride and 25 mM Tris-HCl (pH 8.5) |
