5I2T

Domain characterization of the WD protein Pwp2 and their relevance in ribosome biogenesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	120
Detector technology	PIXEL
Collection date	2014-03-27
Detector	PSI PILATUS 6M
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	70.830, 76.040, 191.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.398 - 2.543
R-factor	0.2153
Rwork	0.213
R-free	0.25210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pbi
RMSD bond length	0.011
RMSD bond angle	1.374
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	BALBES
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.398	2.680
High resolution limit [Å]	2.540	2.540
Rmerge	0.019
Rpim	0.046	0.651
Number of reflections	34863	4990
<I/σ(I)>	12	1.2
Completeness [%]	99.8	100
Redundancy	8.74	9
CC(1/2)	0.998	0.536

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	5 % polyethylene glycol 6000, 0.1 M Magnesium Sulphate