5I2M

CRYSTAL STRUCTURE OF VSV-INDIANA (MUDD-SUMMERS STRAIN) GLYCOPROTEIN UNDER ITS ACIDIC CONFORMATION

Replaces:  2CMZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	C 2 2 21
Unit cell lengths	71.270, 224.200, 375.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.961 - 2.400
R-factor	0.1845
Rwork	0.183
R-free	0.21490
Structure solution method	SIRAS
RMSD bond length	0.008
RMSD bond angle	0.956
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.080	0.550
Number of reflections	117851
<I/σ(I)>	16	3.5
Completeness [%]	99.9	99.4
Redundancy	5.9	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	PROTEIN WAS CRYSTALLIZED FROM 28% PEG 400, 200 MM CACL2, 100 MM HEPES, PH 7.0; THEN SOAKED IN 35% PEG 400, 200 MM CACL2, 100 MM HEPES, PH 7.0