5I2I
Structure of cetuximab Fab with cyclic F3Q variant of the meditope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-07-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.970, 82.500, 211.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.948 - 2.551 |
| R-factor | 0.1618 |
| Rwork | 0.160 |
| R-free | 0.20480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gw1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.875 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 37040 | |
| <I/σ(I)> | 17.8 | 3.68 |
| Completeness [%] | 99.0 | 90.1 |
| Redundancy | 4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M citric acid, 0.1 M sodium phosphate dibasic, 0.5 M potassium phosphate dibasic, 1.6 M sodium phosphate monobasic |
