5I1U
Crystal structure of germacradien-4-ol synthase from Streptomyces citricolor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2014-12-06 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.000064 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.369, 74.127, 82.489 |
Unit cell angles | 90.00, 91.72, 90.00 |
Refinement procedure
Resolution | 55.125 - 1.500 |
R-factor | 0.1577 |
Rwork | 0.157 |
R-free | 0.20040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pentalenene synthase |
RMSD bond length | 0.014 |
RMSD bond angle | 1.347 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1839) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 55.125 |
High resolution limit [Å] | 1.500 |
Number of reflections | 98277 |
<I/σ(I)> | 11.6 |
Completeness [%] | 99.4 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 0.1 M Tris, 2.0 M ammonium sulfate, pH 8.5 |