5I1D

CRYSTAL STRUCTURE OF HUMAN GERMLINE ANTIBODY IGHV3-23/IGKV4-1

Replaces:  3QOS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	CCD
Collection date	2008-04-23
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	62.710, 111.000, 159.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.000
R-factor	0.21632
Rwork	0.216
R-free	0.25132
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b2x 1lve
RMSD bond length	0.008
RMSD bond angle	1.148
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.060
High resolution limit [Å]	2.000	2.000
Rmerge	0.094	0.488
Number of reflections	75540
<I/σ(I)>	21.6	5
Completeness [%]	99.7	96.9
Redundancy	11.6	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	2.0 M AMMONIUM SULFATE, 2% PEG 400, 0.1 M HEPES PH 7.5