5I18

CRYSTAL STRUCTURE OF HUMAN GERMLINE ANTIBODY IGHV1-69/IGKV4-1

Replaces:  3QOT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	95
Detector technology	CCD
Collection date	2008-09-15
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 4 21 2
Unit cell lengths	120.040, 120.040, 64.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.920
R-factor	0.16839
Rwork	0.167
R-free	0.21320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dd8 1lve
RMSD bond length	0.008
RMSD bond angle	1.115
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.970
High resolution limit [Å]	1.920	1.920
Rmerge	0.093	0.231
Number of reflections	35965
<I/σ(I)>	29.2	8.1
Completeness [%]	98.5	82.8
Redundancy	22.3	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	10% PEG 8K, 8% ETHYLENE GLYCOL, 0.1 M HEPES PH 7.5