5I14
Truncated and mutated T4 lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.088, 61.206, 69.806 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.021 - 1.745 |
R-factor | 0.2142 |
Rwork | 0.213 |
R-free | 0.24550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4GBR (N terminal T4L only) |
RMSD bond length | 0.006 |
RMSD bond angle | 0.942 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.021 | 1.808 |
High resolution limit [Å] | 1.745 | 1.745 |
Rmerge | 0.049 | 1.322 |
Number of reflections | 26668 | |
<I/σ(I)> | 22.46 | 1.33 |
Completeness [%] | 99.5 | |
Redundancy | 7.1 | |
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20% PEG 6000, 100mM MES pH 5 |