5I05

Crystal structure of human BMP9 at 1.87 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97949
Spacegroup name	I 41 2 2
Unit cell lengths	71.060, 71.060, 145.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.360 - 1.870
R-factor	0.2155
Rwork	0.213
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kzk
RMSD bond length	0.005
RMSD bond angle	0.734
Data reduction software	XDS ((November 3, 2014))
Data scaling software	XSCALE ((November 3, 2014 BUILT=20141103))
Phasing software	PHASER ((2.56))
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.360	1.920
High resolution limit [Å]	1.870	1.870
Rmerge	0.033	0.790
Number of reflections	15763
<I/σ(I)>	15.95	1.39
Completeness [%]	99.4	99.7
Redundancy	5.7	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	293	1.0 M LiCl, 4% (v/v) PEG6000, 0.1 M NA-CITRATE