5HZM

Human HMT1 hnRNP methyltransferase-like protein 6 (S. cerevisiae)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.25496
Spacegroup name	I 41
Unit cell lengths	94.190, 94.190, 109.609
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.020
R-factor	0.1762
Rwork	0.175
R-free	0.20450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hc4
RMSD bond length	0.011
RMSD bond angle	1.448
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.050
High resolution limit [Å]	2.020	5.480	2.020
Rmerge	0.065	0.028	0.986
Rmeas	0.068
Rpim	0.026
Total number of observations	232791
Number of reflections	31311
<I/σ(I)>	9.7
Completeness [%]	100.0	99.6	100
Redundancy	7.4	7.4	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	10% PEG 8000, 0.1 M Tris-HCL pH8.5