5HZL
Secreted Internalin-like protein Lmo2445 from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 120.350, 120.350, 62.286 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.840 - 2.750 |
| R-factor | 0.19323 |
| Rwork | 0.192 |
| R-free | 0.22571 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.693 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.137 | 0.761 |
| Number of reflections | 26135 | |
| <I/σ(I)> | 13.8 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | Protein: 17.5 mg/ml, 0.5 M NaCl, 10 mM Tris pH 8.3, 1 mM TCEP Crystallization condition: PACT B11 (qiagen), 0.2 M Calcium chloride, 0.1 M MES pH 6, 20% (w/v) PEG 6000 |
