5HYX
Plant peptide hormone receptor RGFR1 in complex with RGF1
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-21 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | H 3 |
Unit cell lengths | 182.335, 182.335, 87.441 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 12.022 - 2.596 |
R-factor | 0.1928 |
Rwork | 0.191 |
R-free | 0.23280 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.421 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 99.000 |
High resolution limit [Å] | 2.596 |
Number of reflections | 33085 |
Completeness [%] | 98.8 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES monohydrate pH 6.0, 22% (v/v) polyethylene glycol (PEG) 400 |