5HYW
The crystal structure of the D3-ASK1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-11 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 43 |
Unit cell lengths | 79.268, 79.268, 327.577 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 81.890 - 3.010 |
R-factor | 0.23921 |
Rwork | 0.235 |
R-free | 0.31193 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hzg |
RMSD bond length | 0.011 |
RMSD bond angle | 1.671 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.890 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 38298 | |
<I/σ(I)> | 12 | |
Completeness [%] | 94.1 | |
Redundancy | 3.69 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 291 | 0.2M Lithium sulfate monohydrate, 0.1M BIS-TRIS pH 6.5, 25% w/v Polyethylene glycol 3350 |