5HY6

Spodoptera frugiperda eukaryotic translation initiation factor EIF5A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-12
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54187
Spacegroup name	P 65
Unit cell lengths	56.780, 56.780, 87.421
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.002 - 2.186
R-factor	0.2077
Rwork	0.205
R-free	0.25950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cpf
RMSD bond length	0.010
RMSD bond angle	1.050
Data reduction software	iMOSFLM
Data scaling software	Aimless (1.10.13)
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.002	2.250
High resolution limit [Å]	2.186	2.190
Rmerge	0.256
Number of reflections	8318
<I/σ(I)>	6.9
Completeness [%]	99.9
Redundancy	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	287.15	Ammonium chloride, MES, PEG 6000