5HXN

Crystal Structure of Z,Z-Farnesyl Diphosphate Synthase (D71M and E75A mutants) from the Wild Tomato Solanum habrochaites

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	93
Detector technology	CCD
Collection date	2013-01-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	51.891, 51.891, 173.286
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.220 - 2.050
R-factor	0.19413
Rwork	0.192
R-free	0.24135
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jp3
RMSD bond length	0.007
RMSD bond angle	1.159
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.120
High resolution limit [Å]	2.050	2.050
Rmerge	0.044	0.495
Number of reflections	15386
<I/σ(I)>	52.3	4.3
Completeness [%]	97.4	94.6
Redundancy	11	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8	291	70% 2-Methyl-2,4-pentanediol (MPD), 0.1 M Tris, pH 8.0.