5HX4
Zinc-Free APOBEC3F Catalytic Domain Crystal Structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.100, 51.270, 99.770 |
| Unit cell angles | 90.00, 90.85, 90.00 |
Refinement procedure
| Resolution | 36.096 - 1.920 |
| R-factor | 0.1863 |
| Rwork | 0.185 |
| R-free | 0.21370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iou |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.951 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2356: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.910 | 2.000 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.054 | 0.910 |
| Number of reflections | 25900 | |
| <I/σ(I)> | 13.1 | 0.8 |
| Completeness [%] | 96.0 | 62.8 |
| Redundancy | 3.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | Tacsimate pH5.0, PEG3350, Glycerol |
