5HX4

Zinc-Free APOBEC3F Catalytic Domain Crystal Structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-31
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	36.100, 51.270, 99.770
Unit cell angles	90.00, 90.85, 90.00

Refinement procedure

Resolution	36.096 - 1.920
R-factor	0.1863
Rwork	0.185
R-free	0.21370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4iou
RMSD bond length	0.011
RMSD bond angle	0.951
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2356: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.910	2.000
High resolution limit [Å]	1.920	1.920
Rmerge	0.054	0.910
Number of reflections	25900
<I/σ(I)>	13.1	0.8
Completeness [%]	96.0	62.8
Redundancy	3.2	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	Tacsimate pH5.0, PEG3350, Glycerol