5HWN
Crystal structure of keto-deoxy-D-galactarate dehydratase complexed with pyruvate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.800, 120.140, 74.070 |
| Unit cell angles | 90.00, 112.01, 90.00 |
Refinement procedure
| Resolution | 48.087 - 1.499 |
| R-factor | 0.1456 |
| Rwork | 0.144 |
| R-free | 0.16950 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.301 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1803) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.090 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.038 | 0.509 |
| Number of reflections | 214991 | |
| <I/σ(I)> | 15.6 | 2.3 |
| Completeness [%] | 97.7 | 97.4 |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | BICINE, sodium formate, PEG MME 5000 |
