5HW4
Crystal structure of Escherichia coli 16S rRNA methyltransferase RsmI in complex with AdoMet
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-01-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.923, 155.270, 54.604 |
| Unit cell angles | 90.00, 93.89, 90.00 |
Refinement procedure
| Resolution | 34.943 - 2.211 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.22950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kwp |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.107 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.063 | 0.535 |
| Number of reflections | 46162 | |
| <I/σ(I)> | 31.5 | |
| Completeness [%] | 93.1 | 97.7 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.2 M DL-Malic acid, 20% PEG 3350 |
