5HVX
Full length Wild-Type Open-form Sodium Channel NavMs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 109.000, 109.000, 210.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.670 - 2.450 |
| R-factor | 0.2087 |
| Rwork | 0.207 |
| R-free | 0.23320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rvy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS (Oct 15, 2015) |
| Data scaling software | XDS (Oct, 15, 2015) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Outer shell | |
| Low resolution limit [Å] | 2.550 |
| High resolution limit [Å] | 2.450 |
| Rmerge | 2.570 |
| <I/σ(I)> | 1.4 |
| Completeness [%] | 100 |
| Redundancy | 29.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.05 M NaCl, 0.1 M MES buffer, pH 6.5, 37.8% v/v polyethylene glycol (PEG) 400 and 4% ethylene glycol |
