5HV2
Rifampin phosphotransferase G527Y mutant from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-04-15 |
| Detector | MAR555 FLAT PANEL |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 58.751, 128.760, 142.392 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.397 - 2.912 |
| R-factor | 0.2168 |
| Rwork | 0.212 |
| R-free | 0.26850 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.503 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 24022 |
| <I/σ(I)> | 14.9 |
| Completeness [%] | 99.1 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.4 | 277 | 19%(w/v) PEG8000, 100 mM Tris-HCl, 200 mM lithium chloride |
