5HUS

Structure of Candida albicans trehalose synthase regulatory protein C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-06
Detector	RAYONIX MX300-HS
Wavelength(s)	1
Spacegroup name	P 65
Unit cell lengths	106.940, 106.940, 41.886
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.973 - 1.751
R-factor	0.188
Rwork	0.186
R-free	0.21680
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.794
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.780
High resolution limit [Å]	1.750	4.750	1.750
Rmerge	0.073	0.059	0.360
Rmeas	0.081	0.066	0.420
Rpim	0.036	0.029	0.212
Total number of observations	149128
Number of reflections	27807
<I/σ(I)>	8.7
Completeness [%]	99.9	98.6	100
Redundancy	5.4	5.2	3.6
CC(1/2)		0.995	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.1 M sodium citrate, 20% PEG 4000, 10% 2-propanol