5HUN

The crystal structure of neuraminidase from A/gyrfalcon/Washington/41088-6/2014 influenza virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-26
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.0
Spacegroup name	I 4
Unit cell lengths	90.652, 90.652, 110.809
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.20262
Rwork	0.201
R-free	0.23704
RMSD bond length	0.013
RMSD bond angle	1.576
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	19878
<I/σ(I)>	47.8
Completeness [%]	99.8
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		293	0.16 M Calcium Acetate, 0.08 M Sodium Cacodylate:HCl pH 6.5, 14.4% (w/v) PEG 8000, 20% (v/v) Glycerol