5HUK
The crystal structure of neuraminidase from A/Northern pintail/Washington/40964/2014 influenza virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-06 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 116.225, 122.789, 176.846 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.450 |
R-factor | 0.15864 |
Rwork | 0.157 |
R-free | 0.18455 |
RMSD bond length | 0.018 |
RMSD bond angle | 2.009 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.450 |
Number of reflections | 91882 |
<I/σ(I)> | 24.9 |
Completeness [%] | 98.3 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 293 | 0.1M Sodium Citrate: Citric acid, pH 5.5, 40% PEG (v/v) 600 |