5HU6
Structure of the T. brucei haptoglobin-haemoglobin receptor bound to human haptolgobin-haemoglobin
Replaces: 4X0IExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.975 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 222.290, 56.560, 66.100 |
| Unit cell angles | 90.00, 92.97, 90.00 |
Refinement procedure
| Resolution | 58.070 - 2.900 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xol |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.102 | 0.420 |
| Number of reflections | 18486 | |
| <I/σ(I)> | 6 | 1.9 |
| Completeness [%] | 99.1 | 98.7 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 12.5% v/v MPD, 0.03 M NaBr, 0.03M NaI, 0.03M NaF, 0.1 M MES/imidazole pH 6.5, 12.5% w/v PEG 1000, 12.5% w/v PEG 3350 |
