5HTF
Crystal Structure of PrsA1 from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-06-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97941 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.310, 84.030, 114.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.782 - 2.100 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.23420 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.934 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | autoSHARP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.782 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.064 | 0.984 |
| Number of reflections | 37408 | |
| <I/σ(I)> | 17.4 | 1.9 |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 5.7 | 5.8 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 278 | 26 mg/mL PrsA1 with a 1:1 mixture of 30% MPD (2-methyl-2,4-pentadiol) 100 mM Na Acetate pH 4.5. |
