5HSZ

Structure of the K. pneumonia SlmA protein bound to the C-terminal tail of the cytoskeletal cell division protein FtsZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 65 2 2
Unit cell lengths	69.261, 69.261, 325.613
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.982 - 2.300
R-factor	0.2207
Rwork	0.216
R-free	0.26740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.534
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	59.982
High resolution limit [Å]	2.300
Rmerge	0.060
Number of reflections	34960
<I/σ(I)>	11.2
Completeness [%]	90.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	600 mM Sodium phosphate, 1200 mM potassium phosphate, 0.1 M imidazole pH 8.0, 0.2 M NaCl