5HSP

MamM CTD M250L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.27
Spacegroup name	C 2 2 21
Unit cell lengths	37.832, 94.914, 53.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.460 - 1.790
R-factor	0.2018
Rwork	0.197
R-free	0.24310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w5x
RMSD bond length	0.012
RMSD bond angle	1.494
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.460
High resolution limit [Å]	1.790
Rmerge	0.110
Number of reflections	9313
<I/σ(I)>	16.5
Completeness [%]	98.7
Redundancy	12.3
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M (NH4)2SO4, 0.1 M BIS-TRIS pH=5.0 and 25% PEG 3350