5HRU

Crystal structure of Plasmodium vivax LDH in complex with a DNA aptamer called pL1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9796
Spacegroup name	P 41 21 2
Unit cell lengths	84.477, 84.477, 209.867
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.960 - 1.710
Rwork	0.160
R-free	0.18450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2a92
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9-1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	25.000	1.740
High resolution limit [Å]	1.710	4.630	1.710
Rmerge	0.103	0.073	0.292
Number of reflections	82531
<I/σ(I)>	15
Completeness [%]	99.8	99.3	99.9
Redundancy	21.4	26.3	15.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.19	PEG-20K, NDSB-195, magnesium chloride