5HRG
The crystal structure of AsfvPolX(D51N mutant):DNA4 binary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 |
Unit cell lengths | 44.060, 51.170, 55.540 |
Unit cell angles | 68.35, 86.25, 88.20 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.19319 |
Rwork | 0.191 |
R-free | 0.23893 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hr9 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.085 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 27077 |
<I/σ(I)> | 6.8 |
Completeness [%] | 93.8 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100mM HEPES pH 7.5, 20% (w/v) PEG 8000, 200mM Ammonium sulfate 10% (v/v) 2-Propanol |