5HRF

The crystal structure of AsfvPolX: DNA5: dGTP ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	58.706, 75.441, 108.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.520 - 2.250
R-factor	0.21494
Rwork	0.212
R-free	0.26325
RMSD bond length	0.006
RMSD bond angle	1.164
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.250
Number of reflections	21028
<I/σ(I)>	27
Completeness [%]	94.2
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2 M Ammonium citrate pH 7.0 20% w/v PEG 3,350