5HRB
The crystal structure of AsfvPolX:DNA1 binary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 43 |
| Unit cell lengths | 77.770, 77.770, 43.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.880 - 1.700 |
| R-factor | 0.17796 |
| Rwork | 0.177 |
| R-free | 0.20219 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hr9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.104 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 27127 |
| <I/σ(I)> | 11.6 |
| Completeness [%] | 99.9 |
| Redundancy | 10.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M HEPES pH 7.5, 10% w/v PEG 3350, 0.2M L-Proline |
