5HR4
Structure of Type IIL restriction-modification enzyme MmeI in complex with DNA has implications for engineering of new specificities
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.877, 95.292, 161.969 |
| Unit cell angles | 72.84, 89.15, 71.62 |
Refinement procedure
| Resolution | 43.047 - 2.596 |
| R-factor | 0.2156 |
| Rwork | 0.215 |
| R-free | 0.24190 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2264: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.596 | 2.596 |
| Rmerge | 0.128 | 0.511 |
| Number of reflections | 91018 | |
| <I/σ(I)> | 11.64 | 2.023 |
| Completeness [%] | 89.3 | 75.2 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 10%(w/v) polyethylene glycol (PEG) 8K,0.1M HEPES pH 7.5, 0.2Mcalcium acetate |
