5HP2

Human Adenosine Deaminase Acting on dsRNA (ADAR2) bound to dsRNA sequence derived from S. cerevisiae BDF2 gene with AU basepair at reaction site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	81.320, 106.680, 120.490
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.526 - 2.983
R-factor	0.1708
Rwork	0.167
R-free	0.24670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zy7
RMSD bond length	0.009
RMSD bond angle	1.359
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.400	3.060
High resolution limit [Å]	2.980	2.980
Rmerge	0.144	0.863
Number of reflections	21442
<I/σ(I)>	10	1.88
Completeness [%]	97.3	90
Redundancy	4.787	4.562
CC(1/2)	0.991

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	0.1 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, 17% PEG10000