5HOZ
Crystal structure of Equine Serum Albumin (ESA) at pH 9.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 |
| Unit cell lengths | 93.825, 93.825, 141.348 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 80.010 - 2.150 |
| R-factor | 0.1975 |
| Rwork | 0.196 |
| R-free | 0.23880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dqf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.458 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 80.010 | 80.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 5.840 | 2.150 |
| Rmerge | 0.050 | 0.025 | 0.737 |
| Number of reflections | 37617 | ||
| <I/σ(I)> | 10.6 | 1.8 | |
| Completeness [%] | 98.1 | 96.3 | 94.3 |
| Redundancy | 5.1 | 4.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 289 | 1 ul of 32 mg/ml protein in 10 mM Tris pH 7.5, 150 mM NaCl was mixed with 1 ul of the well condition (100 mM Tris, 2.4 M Ammonium phosphate, final pH 9.0) and equilibrated against well solution on 15-well Crystallization Plate (Qiagen) |
