5HOP

1.65 Angstrom resolution crystal structure of lmo0182 (residues 1-245) from Listeria monocytogenes EGD-e

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-13
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 43
Unit cell lengths	110.141, 110.141, 45.028
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.450 - 1.650
R-factor	0.16404
Rwork	0.162
R-free	0.19449
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.548
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.087	0.641
Number of reflections	65420
<I/σ(I)>	44.2	3.3
Completeness [%]	99.9	100
Redundancy	7.3	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	295	protein: 15.2 mg/ml in 10 mM Tris-HCl pH 8.3 0.5 mM TCEP crystallization: The PACT Suite C2(#26): 0.1 M PCB buffer pH 5.0 25% (w/v) PEG1500 cryo: crystallization condition