5HOD
Structure of LHX4 transcription factor complexed with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.984 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 40.768, 68.442, 200.464 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.116 - 2.682 |
| R-factor | 0.2347 |
| Rwork | 0.233 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bw5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.884 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.770 |
| High resolution limit [Å] | 2.682 | 2.682 |
| Rmerge | 0.060 | 1.244 |
| Number of reflections | 8245 | |
| <I/σ(I)> | 15.9 | 1.55 |
| Completeness [%] | 98.8 | 91.77 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 6000,Tris buffer, magnesium chloride, MPD |
