5HO1
MamB-CTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.273 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 38.556, 49.828, 95.882 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.940 - 2.530 |
| R-factor | 0.24489 |
| Rwork | 0.240 |
| R-free | 0.29414 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ho5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.178 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.940 | 2.650 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Rmerge | 0.066 | 1.822 |
| Number of reflections | 6527 | |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 99.1 | |
| Redundancy | 6 | |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293.15 | 27% PEG 3350, 0.1 M HEPES pH 7.6, 0.2 M Ammonium sulfate, 5 mM ZnSO4 |
