5HNR
The X-ray structure of octameric human native 5-aminolaevulinic acid dehydratase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-01 |
| Detector | MAR scanner 180 mm plate |
| Wavelength(s) | 0.87 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 125.530, 125.530, 200.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.380 - 2.830 |
| R-factor | 0.1687 |
| Rwork | 0.165 |
| R-free | 0.23785 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aw5 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.655 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.200 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.071 | 0.257 |
| Number of reflections | 18652 | |
| <I/σ(I)> | 6 | 2.9 |
| Completeness [%] | 96.0 | 76.7 |
| Redundancy | 5.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | 10 mg/ml protein concentration in 10 milliM Tris pH 7.4, 10 milliM diothiothreitol and 100 microM zinc chloride. 5 microlitres of this were mixed with an equal volume of 0.1 M MES pH range 6.2 - 6.5, 1.0 - 1.6 M ammonium sulphate and 0 - 10 % dioxane. |
