5HMP
Myosin Vc pre-powerstroke state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.980110 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.780, 66.740, 131.260 |
| Unit cell angles | 90.00, 104.45, 90.00 |
Refinement procedure
| Resolution | 48.500 - 2.397 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.22650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vom |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.998 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.500 | 2.540 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Number of reflections | 67573 | |
| <I/σ(I)> | 13.47 | 1.85 |
| Completeness [%] | 98.3 | 96.4 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 8000, MES, DMSO |
