5HLZ
Structure of Pro-Activin A Complex at 2.85 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 31 |
| Unit cell lengths | 47.227, 47.227, 445.425 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.900 - 2.851 |
| R-factor | 0.2225 |
| Rwork | 0.220 |
| R-free | 0.27360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Structure of the uncleaved growth factor |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.810 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10pre_2084: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.540 | 3.010 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.114 | 0.948 |
| Number of reflections | 26028 | |
| <I/σ(I)> | 9.7 | 1.6 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.1 | 4.9 |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 292 | 20% w/v polyethylene glycol 3350, 0.2 M calcium chloride; cryo: 15% v/v PEG 400 added |
