5HLE

Structural basis of backwards motion in kinesin-14: minus-end directed nKn664 in the ADP state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	93
Detector technology	CCD
Collection date	2015-07-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	112.858, 112.858, 72.110
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.548 - 2.900
R-factor	0.2185
Rwork	0.211
R-free	0.28720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bg2
RMSD bond length	0.011
RMSD bond angle	1.573
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.850
Number of reflections	12353
<I/σ(I)>	18.9
Completeness [%]	100.0
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	PEG 3350, Ammonium sulfate, HEPES Benzamidine-HCl, ADP