5HKK
Caldalaklibacillus thermarum F1-ATPase (wild type)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 147.820, 130.790, 210.390 |
| Unit cell angles | 90.00, 107.97, 90.00 |
Refinement procedure
| Resolution | 62.160 - 3.000 |
| R-factor | 0.2051 |
| Rwork | 0.203 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ck3 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.567 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0144) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.300 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.108 | 0.444 |
| Number of reflections | 145626 | |
| <I/σ(I)> | 11.6 | 3 |
| Completeness [%] | 95.6 | 88.9 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 295 | PEG 4600, PEG 1500, ADP, Magnesium chloride |
