5HKD
Bacterial sodium channel neck 7G mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2014-03-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0332 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 137.100, 137.990, 173.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 3.800 |
R-factor | 0.27467 |
Rwork | 0.273 |
R-free | 0.31198 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lto |
RMSD bond length | 0.012 |
RMSD bond angle | 1.894 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.600 | |
High resolution limit [Å] | 3.800 | 3.800 |
Rmerge | 0.660 | |
Number of reflections | 18097 | |
<I/σ(I)> | 6.22 | |
Completeness [%] | 97.4 | |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277 | 30% PEG400, 20 mM MES, pH 5.8 |