5HJR
Murine endoplasmic reticulum alpha-glucosidase II with bound covalent intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.07227 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.512, 174.664, 63.714 |
| Unit cell angles | 90.00, 91.31, 90.00 |
Refinement procedure
| Resolution | 103.480 - 2.400 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.20200 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 174.660 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 87472 | |
| <I/σ(I)> | 7.6 | 1.2 |
| Completeness [%] | 99.3 | 98.8 |
| Redundancy | 6.9 | 7 |
| CC(1/2) | 0.364 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.25 | 291 | 32 % Morpheus ethylene glycol/PEG 8000 mix, 0.05 M Morpheus carboxylic acids mix, 0.1 M Morpheus buffer system 1 |
