5HJL
Crystal structure of class I tagatose 1,6-bisphosphate aldolase LacD from Streptococcus porcinus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.7101 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.660, 93.750, 88.110 |
| Unit cell angles | 90.00, 120.58, 90.00 |
Refinement procedure
| Resolution | 46.870 - 3.000 |
| Rwork | 0.174 |
| R-free | 0.22920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iv3 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.914 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.880 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.296 | 1.130 |
| Number of reflections | 14194 | |
| <I/σ(I)> | 5.9 | 2.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.5 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Small crystals grow in 25% PEG 3350, 0.1M bistris pH 5.5, 0.2 M Ammonium Sulfate. The crystal used for data collection was obtained after micro seeding in the same condition. |
