5HJJ

Crystal Structure of Pyrococcus abyssi Trm5a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-06
Detector	OXFORD ONYX CCD
Wavelength(s)	1.5
Spacegroup name	P 21 21 21
Unit cell lengths	52.832, 54.324, 129.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.559 - 2.000
R-factor	0.1991
Rwork	0.197
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hjk
RMSD bond length	0.004
RMSD bond angle	0.886
Data reduction software	CrysalisPro
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.800	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.136	0.740
Number of reflections	25714
<I/σ(I)>	7.9	5.1
Completeness [%]	99.5	98.7
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	10-15% PEG 3350, 100 mM HEPES, pH7.5, 100 mM Ca(OAc)2 and 100 mM KCl